ABSTRACT
OBJECTIVE：To study the anti-inflammatory effects of Yuyang capsule on bacterial dermatitis model rats and its effect on TLR/NF-κ B pathway. METHODS：Minimum inhibition concentration （MIC）and minimal bactericidal concentration （MBC）of Yuyang capsule against Staphylococcus aureus were determined by microdilution test. Totally 50 SD rats were randomly divided into model group ，positive control group （amoxicillin and clavulanate potassium ，120 mg/kg as amoxicillin ），Yuyang capsules high-dose ，medium-dose and low-dose groups （according to MIC ），with 10 rats in each group. The model of bacterial dermatitis was established by using the burned skin of rats infected with S. aureus . 24 h after modeling ，administration groups were intragastrically given the corresponding drug ，and model group was intragastrically given the same amount of normal saline ，once a day，for consecutive 7 days. The skin healing rate was calculated on the 1st，3rd，5th and 7th day of administration ，and the scab formation，decrustation and healing were recorded. The contents of IL- 1β，IL-6，IL-10，TNF-α，hydroxyproline（HYP），collagen Ⅰ（Col Ⅰ）and Col Ⅲ in skin tissue were detected by ELISA. Morphology changes of skin tissues were observed by HE staining. The ultrastructure was observed by transmission electron microscope. Protein expression of TLR 2，TLR4 and p-NF-κB p65 were detected by Western blotting assay. RESULTS ：MIC and MBC of Yuyang capsule were 25 mg/mL and 50 mg/mL，respectively. Dose of Yuyang capsules high-dose ，medium-dose and low-dose groups were set at 600，300，150 mg/kg. Compared with model group，scab appeared on the injured skin 3 days after administration in Yuyang capsule high-dose and medium-dose groups ，and decrustation appeared on the injured skin of part mice 5-7 days after administration ；the skin healing rate of the positive control group，Yuyang capsule high-dose and medium-dose groups were all significantly increased at each time point. The contents of IL- 1 β，IL-6，IL-10 and TNF-α，pathological score ，protein expression of TLR 2，TLR4 and p-NF-κB p65 were decreased significantly （P＜0.05 or P＜0.01）；the pathological changes such as inflammatory cell infiltration in skin tissue were improved. The contents of HYP，Col Ⅰ and Col Ⅲ were increased significantly in positive control group and Yuyang capsule high-dose group （P＜0.05 or P＜0.01）. There was no statistical significance in most above indexes in positive control group ，Yuyang capsule high-dose and medium-dose groups （P＞0.05）. CONCLUSIONS ：Yuyang capsule can promote skin healing of bacterial dermatitis model rats and shows certain anti-inflammatory effects ；the mechanism may be related to inhibiting TLR/NF-κB signaling pathway and reducing inflammatory response.
ABSTRACT
Background: Skin diseases are common in infancy. This study aimed to determine to prevalence of various dermatoses in infancy in Uttarakhand. Objectives: To determine the prevalence of different dermatologic diseases in infant in Uttarakhand. Methods: A prospective study was carried out for one year from 06 Sep 2013 to 05 Sep 2014, to study prevalence and patterns of dermatological lesions in infants, a tertiary care health facility in Uttarakhand. A total of 234 infants of both sexes were seen during this period. Results: Of 234 patients 58.9% were males. Noninfectious dermatitis was the largest group of skin disorders in infancy, constituting 35.89% of total cases. Seborrheic dermatitis was commonest type of non-infectious dermatitis in infancy (42.85%) followed by atopic dermatitis (19.04%), Ptyriasis alba (14.28 %), contact and diaper dermatitis (7.14% each). Seborrheic dermatitis did not display any gender or seasonal bias. Infectious/ parasitic infestations formed second largest group of dermatoses in infants (26.92%) with commonest being fungal infections (49% cases of infectious group) followed by parasitic infestations (26.98%). Bacterial and viral infections were formed only 12.69% and 11.11% of infectious group cases. Nonfungal and nonparasitic infections showed higher incidence in summers. Conclusion: Out of 234 infants who attended paediatric dermatology clinic, major dermatoses were of noninfectious origin with seborrheic dermatitis forming largest group. Infectious etiology dermatoses constituted second major group with fungal and parasitic etiology. Nonfungal and non-parasitic dermatoses showed higher prevalence in summer months.